lifr alpha antibody Search Results


facs buffer  (R&D Systems)
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R&D Systems facs buffer
Facs Buffer, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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antibody against lifr  (Bio-Techne corporation)
90
Bio-Techne corporation antibody against lifr
KEY RESOURCES TABLE
Antibody Against Lifr, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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western blotting  (R&D Systems)
93
R&D Systems western blotting
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Western Blotting, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lifralpha  (R&D Systems)
92
R&D Systems lifralpha
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Lifralpha, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lifr  (Novus Biologicals)
90
Novus Biologicals lifr
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Lifr, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phycoerythrin pe conjugated mouse anti human lifr antibodies  (R&D Systems)
90
R&D Systems phycoerythrin pe conjugated mouse anti human lifr antibodies
Influence of Musashi ( MSI ) RNA-binding protein knockdown on leukemia inhibitory factor receptor <t>(LIFR)</t> expression, cell migration and invasiveness in triple-negative MDA-MB-231 cells. 5A1 : The LIFR is downregulated in MSI knockdown cells both in qPCR and in flow cytometric measurements (mean x being the mean fluorescence intensity of the respective sample) with a representative histogram including respective isotypes (unspecific antibodies of the same subclass that show low fluorescence intensity and no discernible difference between samples, thus indicating that changes are due to specific antibody binding) in 5A2 (on a logarithmic x scale). 5B1 : Cell migration distance increases subsequent to MSI knockdown compared to controls (ctrl): Cell tracking of control cells ( 5B2 ) and MSI knockdown cells ( 5B3 ) demonstrates higher motility in the latter. 5C1 : Cell invasiveness is strongly enhanced after MSI silencing with representative pictures of invasive cells for controls ( 5C2 ) and MSI knockdown cells ( 5C3 ). Respective staining was performed with 1% Toluidine blue. Cells were transfected with a control siRNA and MSI-1 and MSI-2 siRNA, respectively, as detailed in the Methods section (at least n = 3, * p < 0.05, ** p < 0.01, *** p < 0.001, error bars indicate s.e.m.).
Phycoerythrin Pe Conjugated Mouse Anti Human Lifr Antibodies, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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primary antibody  (R&D Systems)
93
R&D Systems primary antibody
Influence of Musashi ( MSI ) RNA-binding protein knockdown on leukemia inhibitory factor receptor <t>(LIFR)</t> expression, cell migration and invasiveness in triple-negative MDA-MB-231 cells. 5A1 : The LIFR is downregulated in MSI knockdown cells both in qPCR and in flow cytometric measurements (mean x being the mean fluorescence intensity of the respective sample) with a representative histogram including respective isotypes (unspecific antibodies of the same subclass that show low fluorescence intensity and no discernible difference between samples, thus indicating that changes are due to specific antibody binding) in 5A2 (on a logarithmic x scale). 5B1 : Cell migration distance increases subsequent to MSI knockdown compared to controls (ctrl): Cell tracking of control cells ( 5B2 ) and MSI knockdown cells ( 5B3 ) demonstrates higher motility in the latter. 5C1 : Cell invasiveness is strongly enhanced after MSI silencing with representative pictures of invasive cells for controls ( 5C2 ) and MSI knockdown cells ( 5C3 ). Respective staining was performed with 1% Toluidine blue. Cells were transfected with a control siRNA and MSI-1 and MSI-2 siRNA, respectively, as detailed in the Methods section (at least n = 3, * p < 0.05, ** p < 0.01, *** p < 0.001, error bars indicate s.e.m.).
Primary Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse anti human lif monoclonal antibody  (R&D Systems)
90
R&D Systems mouse anti human lif monoclonal antibody
Influence of Musashi ( MSI ) RNA-binding protein knockdown on leukemia inhibitory factor receptor <t>(LIFR)</t> expression, cell migration and invasiveness in triple-negative MDA-MB-231 cells. 5A1 : The LIFR is downregulated in MSI knockdown cells both in qPCR and in flow cytometric measurements (mean x being the mean fluorescence intensity of the respective sample) with a representative histogram including respective isotypes (unspecific antibodies of the same subclass that show low fluorescence intensity and no discernible difference between samples, thus indicating that changes are due to specific antibody binding) in 5A2 (on a logarithmic x scale). 5B1 : Cell migration distance increases subsequent to MSI knockdown compared to controls (ctrl): Cell tracking of control cells ( 5B2 ) and MSI knockdown cells ( 5B3 ) demonstrates higher motility in the latter. 5C1 : Cell invasiveness is strongly enhanced after MSI silencing with representative pictures of invasive cells for controls ( 5C2 ) and MSI knockdown cells ( 5C3 ). Respective staining was performed with 1% Toluidine blue. Cells were transfected with a control siRNA and MSI-1 and MSI-2 siRNA, respectively, as detailed in the Methods section (at least n = 3, * p < 0.05, ** p < 0.01, *** p < 0.001, error bars indicate s.e.m.).
Mouse Anti Human Lif Monoclonal Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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goat antihuman leukemia inhibitory factor receptor lifr  (R&D Systems)
92
R&D Systems goat antihuman leukemia inhibitory factor receptor lifr
Influence of Musashi ( MSI ) RNA-binding protein knockdown on leukemia inhibitory factor receptor <t>(LIFR)</t> expression, cell migration and invasiveness in triple-negative MDA-MB-231 cells. 5A1 : The LIFR is downregulated in MSI knockdown cells both in qPCR and in flow cytometric measurements (mean x being the mean fluorescence intensity of the respective sample) with a representative histogram including respective isotypes (unspecific antibodies of the same subclass that show low fluorescence intensity and no discernible difference between samples, thus indicating that changes are due to specific antibody binding) in 5A2 (on a logarithmic x scale). 5B1 : Cell migration distance increases subsequent to MSI knockdown compared to controls (ctrl): Cell tracking of control cells ( 5B2 ) and MSI knockdown cells ( 5B3 ) demonstrates higher motility in the latter. 5C1 : Cell invasiveness is strongly enhanced after MSI silencing with representative pictures of invasive cells for controls ( 5C2 ) and MSI knockdown cells ( 5C3 ). Respective staining was performed with 1% Toluidine blue. Cells were transfected with a control siRNA and MSI-1 and MSI-2 siRNA, respectively, as detailed in the Methods section (at least n = 3, * p < 0.05, ** p < 0.01, *** p < 0.001, error bars indicate s.e.m.).
Goat Antihuman Leukemia Inhibitory Factor Receptor Lifr, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phycoerythrin pe conjugated monoclonal mouse antihuman α sma antibody  (R&D Systems)
91
R&D Systems phycoerythrin pe conjugated monoclonal mouse antihuman α sma antibody
Influence of Musashi ( MSI ) RNA-binding protein knockdown on leukemia inhibitory factor receptor <t>(LIFR)</t> expression, cell migration and invasiveness in triple-negative MDA-MB-231 cells. 5A1 : The LIFR is downregulated in MSI knockdown cells both in qPCR and in flow cytometric measurements (mean x being the mean fluorescence intensity of the respective sample) with a representative histogram including respective isotypes (unspecific antibodies of the same subclass that show low fluorescence intensity and no discernible difference between samples, thus indicating that changes are due to specific antibody binding) in 5A2 (on a logarithmic x scale). 5B1 : Cell migration distance increases subsequent to MSI knockdown compared to controls (ctrl): Cell tracking of control cells ( 5B2 ) and MSI knockdown cells ( 5B3 ) demonstrates higher motility in the latter. 5C1 : Cell invasiveness is strongly enhanced after MSI silencing with representative pictures of invasive cells for controls ( 5C2 ) and MSI knockdown cells ( 5C3 ). Respective staining was performed with 1% Toluidine blue. Cells were transfected with a control siRNA and MSI-1 and MSI-2 siRNA, respectively, as detailed in the Methods section (at least n = 3, * p < 0.05, ** p < 0.01, *** p < 0.001, error bars indicate s.e.m.).
Phycoerythrin Pe Conjugated Monoclonal Mouse Antihuman α Sma Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lifr antibody  (R&D Systems)
86
R&D Systems lifr antibody
Influence of Musashi ( MSI ) RNA-binding protein knockdown on leukemia inhibitory factor receptor <t>(LIFR)</t> expression, cell migration and invasiveness in triple-negative MDA-MB-231 cells. 5A1 : The LIFR is downregulated in MSI knockdown cells both in qPCR and in flow cytometric measurements (mean x being the mean fluorescence intensity of the respective sample) with a representative histogram including respective isotypes (unspecific antibodies of the same subclass that show low fluorescence intensity and no discernible difference between samples, thus indicating that changes are due to specific antibody binding) in 5A2 (on a logarithmic x scale). 5B1 : Cell migration distance increases subsequent to MSI knockdown compared to controls (ctrl): Cell tracking of control cells ( 5B2 ) and MSI knockdown cells ( 5B3 ) demonstrates higher motility in the latter. 5C1 : Cell invasiveness is strongly enhanced after MSI silencing with representative pictures of invasive cells for controls ( 5C2 ) and MSI knockdown cells ( 5C3 ). Respective staining was performed with 1% Toluidine blue. Cells were transfected with a control siRNA and MSI-1 and MSI-2 siRNA, respectively, as detailed in the Methods section (at least n = 3, * p < 0.05, ** p < 0.01, *** p < 0.001, error bars indicate s.e.m.).
Lifr Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lifr alpha antibody  (Bio-Techne corporation)
90
Bio-Techne corporation lifr alpha antibody
Influence of Musashi ( MSI ) RNA-binding protein knockdown on leukemia inhibitory factor receptor <t>(LIFR)</t> expression, cell migration and invasiveness in triple-negative MDA-MB-231 cells. 5A1 : The LIFR is downregulated in MSI knockdown cells both in qPCR and in flow cytometric measurements (mean x being the mean fluorescence intensity of the respective sample) with a representative histogram including respective isotypes (unspecific antibodies of the same subclass that show low fluorescence intensity and no discernible difference between samples, thus indicating that changes are due to specific antibody binding) in 5A2 (on a logarithmic x scale). 5B1 : Cell migration distance increases subsequent to MSI knockdown compared to controls (ctrl): Cell tracking of control cells ( 5B2 ) and MSI knockdown cells ( 5B3 ) demonstrates higher motility in the latter. 5C1 : Cell invasiveness is strongly enhanced after MSI silencing with representative pictures of invasive cells for controls ( 5C2 ) and MSI knockdown cells ( 5C3 ). Respective staining was performed with 1% Toluidine blue. Cells were transfected with a control siRNA and MSI-1 and MSI-2 siRNA, respectively, as detailed in the Methods section (at least n = 3, * p < 0.05, ** p < 0.01, *** p < 0.001, error bars indicate s.e.m.).
Lifr Alpha Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


KEY RESOURCES TABLE

Journal: Cell stem cell

Article Title: LIF signaling regulates outer radial glial to interneuron fate during human cortical development

doi: 10.1016/j.stem.2023.08.009

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: The conjugated antibody against LIFR (biotechne, #FAB249A) was added directly to the suspended cells (100 μl per 1 ml FACS buffer) and incubated for 30 minutes.

Techniques: Recombinant, Multiplex Assay, Software

Influence of Musashi ( MSI ) RNA-binding protein knockdown on leukemia inhibitory factor receptor (LIFR) expression, cell migration and invasiveness in triple-negative MDA-MB-231 cells. 5A1 : The LIFR is downregulated in MSI knockdown cells both in qPCR and in flow cytometric measurements (mean x being the mean fluorescence intensity of the respective sample) with a representative histogram including respective isotypes (unspecific antibodies of the same subclass that show low fluorescence intensity and no discernible difference between samples, thus indicating that changes are due to specific antibody binding) in 5A2 (on a logarithmic x scale). 5B1 : Cell migration distance increases subsequent to MSI knockdown compared to controls (ctrl): Cell tracking of control cells ( 5B2 ) and MSI knockdown cells ( 5B3 ) demonstrates higher motility in the latter. 5C1 : Cell invasiveness is strongly enhanced after MSI silencing with representative pictures of invasive cells for controls ( 5C2 ) and MSI knockdown cells ( 5C3 ). Respective staining was performed with 1% Toluidine blue. Cells were transfected with a control siRNA and MSI-1 and MSI-2 siRNA, respectively, as detailed in the Methods section (at least n = 3, * p < 0.05, ** p < 0.01, *** p < 0.001, error bars indicate s.e.m.).

Journal: International Journal of Molecular Sciences

Article Title: Knockdown of Musashi RNA Binding Proteins Decreases Radioresistance but Enhances Cell Motility and Invasion in Triple-Negative Breast Cancer

doi: 10.3390/ijms21062169

Figure Lengend Snippet: Influence of Musashi ( MSI ) RNA-binding protein knockdown on leukemia inhibitory factor receptor (LIFR) expression, cell migration and invasiveness in triple-negative MDA-MB-231 cells. 5A1 : The LIFR is downregulated in MSI knockdown cells both in qPCR and in flow cytometric measurements (mean x being the mean fluorescence intensity of the respective sample) with a representative histogram including respective isotypes (unspecific antibodies of the same subclass that show low fluorescence intensity and no discernible difference between samples, thus indicating that changes are due to specific antibody binding) in 5A2 (on a logarithmic x scale). 5B1 : Cell migration distance increases subsequent to MSI knockdown compared to controls (ctrl): Cell tracking of control cells ( 5B2 ) and MSI knockdown cells ( 5B3 ) demonstrates higher motility in the latter. 5C1 : Cell invasiveness is strongly enhanced after MSI silencing with representative pictures of invasive cells for controls ( 5C2 ) and MSI knockdown cells ( 5C3 ). Respective staining was performed with 1% Toluidine blue. Cells were transfected with a control siRNA and MSI-1 and MSI-2 siRNA, respectively, as detailed in the Methods section (at least n = 3, * p < 0.05, ** p < 0.01, *** p < 0.001, error bars indicate s.e.m.).

Article Snippet: CD44 and LIFR positivity were analyzed 48 h after transfection using CD44 APC (BD Pharmingen, Franklin Lakes, NJ, USA) or phycoerythrin (PE) conjugated mouse anti-human LIFR antibodies (R&D Systems, Minneapolis, MN, USA) and their corresponding isotype controls (APC isotype from BD Pharmingen, PE isotype from R&D Systems).

Techniques: RNA Binding Assay, Knockdown, Expressing, Migration, Fluorescence, Binding Assay, Cell Tracking Assay, Control, Staining, Transfection